在灾难受害者鉴定中消除折衷

体彩大乐透预测推荐号:灾难受害者的鉴定

超级大乐透中奖规则 www.slf2t.cn 对于大规模灾难的受害者,当传统方法无法使用或不能产生结论性的结果时,可使用遗传分析来鉴定。由于新一代测序(NGS)在能够分析的位点数量或每个等位基因的长度上没有相关限制,故能从证据或已知的参考样本中提取出最大量的信息。

传统的灾难受害者鉴定方法可能包括常规方法,如指纹和牙齿。实验室也可使用分子方法,如通过毛细管电泳的DNA分析和mtDNA测序。在大规模死亡事故发生后处理的样本类型可能包括碎骨、组织、各种含有DNA的私人物品,以及亲属的口腔拭子。 灾难受害者鉴定中,DNA检测的目标是从高度受损的样本中提取出尽可能多的遗传信息。对于使用毛细管电泳的传统DNA检测,选择一种分析往往会排除另一种的开展。分析人员必须评估证据材料,并决定开展哪种分析方法

Disaster Victim Identification

The goal of DNA testing for disaster victim identification is to extract as much genetic information as possible from highly compromised samples. With traditional DNA testing using capillary electrophoresis, choosing one assay often precludes the ability to perform another. Analysts must evaluate the evidentiary material and decide which assay method to perform.

With Illumina NGS technology, these tradeoffs are eliminated. NGS systems such as the MiSeq FGx Forensic Genomics System have no relevant limit on the number of loci that can be analyzed on the same sample in the same run. Every locus can be assayed, including core sets of autosomal, Y and X STRs, several categories of SNPs, the mtDNA genome, and other classes of polymorphisms.

For disaster victim identification, Illumina offers a complete, fully validated DNA-to-data solution designed for forensic genomics. Our recommended workflow on the MiSeq FGx System utilizes the ForenSeq DNA Signature Prep Kit or the Nextera XT DNA Library Prep Kit.

Achieve high resolution and exceptional accuracy from as little as 1 ng of DNA—Even with complex mixtures or degraded DNA. The inherent sensitivity of Illumina chemistry helps detect minor components that might go undetected by conventional STR and CE analysis.

Click on the below to view products for each workflow step.

DNA Extraction and Quantification:
We recommend using your current DNA extraction and quantitation methods.

 

Library Preparation:
ForenSeq DNA Signature Prep Kit (for nuclear DNA)

Assay approximately 200 genetic markers in a single test. Includes all reagents to prepare sequencing libraries from forensic DNA samples.

Nextera XT DNA Library Prep Kit (for mtDNA)

Prepare sequencing-ready libraries for small genomes like mitochondrial DNA in less than 90 minutes.

MiSeq FGx Forensic Genomics System

First fully validated sequencing system for forensic genomics.

MiSeq FGx Reagent Kit

Pre-filled, ready-to-use reagent cartridges, specifically designed for the MiSeq FGx System. Use for sequencing nuclear DNA libraries.

MiSeq Reagent Kit v2

Pre-filled, ready-to-use reagent cartridges, for running the MiSeq FGx System in RUO mode. Use for sequencing mtDNA libraries.

ForenSeq Universal Analysis Software

Analyze and interpret a wide range of forensics casework and database samples.

MyFLq BaseSpace App

My F(orensic) L(oci) q(ueries) - Open source, straightforward analysis tool for forensic DNA samples and profile generation.

mtDNA Variant Processor BaseSpace App

Enables streamlined variant analysis of d-loop and whole mitochondrial DNA sequence data.

mtDNA Variant Analyzer BaseSpace App

Enables simplified visualization of d-loop and whole mitochondrial DNA sequence data.

有了Illumina的NGS技术,就不再需要这种折衷。NGS系统,如MiSeq FGx Forensic Genomics System,在能够分析的位点数量或每个等位基因的长度上没有相关限制。每个位点都能分析,包括常染色体以及Y和X STR的核心组合、几类SNP、mtDNA基因组,以及其他种类的多态性。

这种法医基因组学方法能够在单次MiSeq运行中,从证据样本或已知的参考样本中产生尽可能多的信息。对于灾难受害者的鉴定,Illumina建议使用MiSeq FGx以及ForenSeq DNA Signature Prep Kit 和 Nextera XT DNA Sample Prep Kit。

单击下面的 可查看每个工作流程步骤的产品。


DNA提取 & 定量

我们建议您使用目前的DNA提取和定量方法;Illumina不提供这一步骤的产品。

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